l type ca2 antibody Search Results


cav1  (Alomone Labs)
96
Alomone Labs cav1
Cav1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti α2δ 1  (Alomone Labs)
93
Alomone Labs rabbit anti α2δ 1
TSP-receptor <t>α2δ-1</t> is synaptically expressed in the retina, and its expression is reduced in RCS rats. A, Representative images of the retina stained for α2δ-1 from LE (healthy) and RCS (degenerative) retinas at P14 and (B) P30. C, Quantitative staining intensity analysis demonstrated that α2δ-1 expression was reduced in RCS rat as early as P14. D, α2δ-1 was enriched in both the OPL and IPL, and the expression gap became more distinct at P30. Representative images of the (E) OPL and (F and G) IPL with the synapses labeled for Bassoon (F, green), VGluT1 (G, green), α2δ-1 (red), and NR1 (blue) from LE retina on P21 demonstrated postsynaptic expression of α2δ-1. Representative images of the (H) OPL and (J) IPL synapses labeled for Bassoon (green) and α2δ-1 (red) from LE (healthy) and RCS (degenerative) retinas on P21. Quantification of α2δ-1-containing synapses formed in the (I) OPL and (K) IPL reveals that the number of α2δ-1 synapse is already reduced in RCS rat by P21. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.
Rabbit Anti α2δ 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti α2δ 1/product/Alomone Labs
Average 93 stars, based on 1 article reviews
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polyclonal antibody bab  (Boster Bio)
90
Boster Bio polyclonal antibody bab
TSP-receptor <t>α2δ-1</t> is synaptically expressed in the retina, and its expression is reduced in RCS rats. A, Representative images of the retina stained for α2δ-1 from LE (healthy) and RCS (degenerative) retinas at P14 and (B) P30. C, Quantitative staining intensity analysis demonstrated that α2δ-1 expression was reduced in RCS rat as early as P14. D, α2δ-1 was enriched in both the OPL and IPL, and the expression gap became more distinct at P30. Representative images of the (E) OPL and (F and G) IPL with the synapses labeled for Bassoon (F, green), VGluT1 (G, green), α2δ-1 (red), and NR1 (blue) from LE retina on P21 demonstrated postsynaptic expression of α2δ-1. Representative images of the (H) OPL and (J) IPL synapses labeled for Bassoon (green) and α2δ-1 (red) from LE (healthy) and RCS (degenerative) retinas on P21. Quantification of α2δ-1-containing synapses formed in the (I) OPL and (K) IPL reveals that the number of α2δ-1 synapse is already reduced in RCS rat by P21. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.
Polyclonal Antibody Bab, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cav1 2  (Alomone Labs)
94
Alomone Labs cav1 2
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cav1 2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
cav1 2 - by Bioz Stars, 2026-02
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acc 003 ag  (Alomone Labs)
92
Alomone Labs acc 003 ag
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Acc 003 Ag, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acc 003 ag/product/Alomone Labs
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acc 003 ag - by Bioz Stars, 2026-02
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s0471 galectin 3 inhibitor cpd47 merck n a cacng1 blocking peptide alomone labs  (Alomone Labs)
94
Alomone Labs s0471 galectin 3 inhibitor cpd47 merck n a cacng1 blocking peptide alomone labs
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
S0471 Galectin 3 Inhibitor Cpd47 Merck N A Cacng1 Blocking Peptide Alomone Labs, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
s0471 galectin 3 inhibitor cpd47 merck n a cacng1 blocking peptide alomone labs - by Bioz Stars, 2026-02
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rabbit antibodies against voltage-gated ca 2+ channel (p/q-type, α 1a subunit)  (Synaptic Systems)
90
Synaptic Systems rabbit antibodies against voltage-gated ca 2+ channel (p/q-type, α 1a subunit)
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Rabbit Antibodies Against Voltage Gated Ca 2+ Channel (P/Q Type, α 1a Subunit), supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antibodies against voltage-gated ca 2+ channel (p/q-type, α 1a subunit)/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
rabbit antibodies against voltage-gated ca 2+ channel (p/q-type, α 1a subunit) - by Bioz Stars, 2026-02
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polyclonal guinea pig ca 2+ channel n-type alpha-1b channel 152305 antibody  (Synaptic Systems)
90
Synaptic Systems polyclonal guinea pig ca 2+ channel n-type alpha-1b channel 152305 antibody
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Polyclonal Guinea Pig Ca 2+ Channel N Type Alpha 1b Channel 152305 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal guinea pig ca 2+ channel n-type alpha-1b channel 152305 antibody/product/Synaptic Systems
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anti - cav 2.1 alpha-1a rabbit polyclonal  (Synaptic Systems)
90
Synaptic Systems anti - cav 2.1 alpha-1a rabbit polyclonal
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Anti Cav 2.1 Alpha 1a Rabbit Polyclonal, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti - cav 2.1 alpha-1a rabbit polyclonal/product/Synaptic Systems
Average 90 stars, based on 1 article reviews
anti - cav 2.1 alpha-1a rabbit polyclonal - by Bioz Stars, 2026-02
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na + /ca 2+ -exchanger type-1  (Swant)
90
Swant na + /ca 2+ -exchanger type-1
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Na + /Ca 2+ Exchanger Type 1, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/na + /ca 2+ -exchanger type-1/product/Swant
Average 90 stars, based on 1 article reviews
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anti serca2 antibody  (Boster Bio)
93
Boster Bio anti serca2 antibody
NHE1 colocalisation with <t>CaV1.2</t> ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.
Anti Serca2 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti serca2 antibody/product/Boster Bio
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Image Search Results


TSP-receptor α2δ-1 is synaptically expressed in the retina, and its expression is reduced in RCS rats. A, Representative images of the retina stained for α2δ-1 from LE (healthy) and RCS (degenerative) retinas at P14 and (B) P30. C, Quantitative staining intensity analysis demonstrated that α2δ-1 expression was reduced in RCS rat as early as P14. D, α2δ-1 was enriched in both the OPL and IPL, and the expression gap became more distinct at P30. Representative images of the (E) OPL and (F and G) IPL with the synapses labeled for Bassoon (F, green), VGluT1 (G, green), α2δ-1 (red), and NR1 (blue) from LE retina on P21 demonstrated postsynaptic expression of α2δ-1. Representative images of the (H) OPL and (J) IPL synapses labeled for Bassoon (green) and α2δ-1 (red) from LE (healthy) and RCS (degenerative) retinas on P21. Quantification of α2δ-1-containing synapses formed in the (I) OPL and (K) IPL reveals that the number of α2δ-1 synapse is already reduced in RCS rat by P21. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.

Journal: The Journal of Neuroscience

Article Title: Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity

doi: 10.1523/JNEUROSCI.1532-17.2018

Figure Lengend Snippet: TSP-receptor α2δ-1 is synaptically expressed in the retina, and its expression is reduced in RCS rats. A, Representative images of the retina stained for α2δ-1 from LE (healthy) and RCS (degenerative) retinas at P14 and (B) P30. C, Quantitative staining intensity analysis demonstrated that α2δ-1 expression was reduced in RCS rat as early as P14. D, α2δ-1 was enriched in both the OPL and IPL, and the expression gap became more distinct at P30. Representative images of the (E) OPL and (F and G) IPL with the synapses labeled for Bassoon (F, green), VGluT1 (G, green), α2δ-1 (red), and NR1 (blue) from LE retina on P21 demonstrated postsynaptic expression of α2δ-1. Representative images of the (H) OPL and (J) IPL synapses labeled for Bassoon (green) and α2δ-1 (red) from LE (healthy) and RCS (degenerative) retinas on P21. Quantification of α2δ-1-containing synapses formed in the (I) OPL and (K) IPL reveals that the number of α2δ-1 synapse is already reduced in RCS rat by P21. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.

Article Snippet: Primary antibodies (mouse anti-Bassoon 1:500 [RRID: AB_10618753 , ADI-VAM-PS003-F, Enzo], rabbit anti-mGluR6 1:150 [RRID:not applicable, RA13105, Neuromics], guinea pig anti-VGlut1 1:750 [AB5905, Millipore], rabbit anti-PSD95 1:500 [RRID: AB_87705 , 51-6900, Invitrogen], mouse anti-Gephyrin 1:250 [RRID: AB_1279448 , 147-021, Synaptic Systems], goat anti-TSP1 1:200 [RRID: AB_2201958 , AF3074, R&D Systems], goat-anti-TSP2 1:200 [RRID: AB_2202068 , AF1635, R&D Systems], mouse anti-glutamine synthetase 1:1000 [RRID: AB_397879 , 610517, BD Biosciences], rabbit anti-SOX9 1:4000 [RRID: AB_2239761 , AB5535, Millipore], goat anti-ChAT[RRID: AB_11214092 , AB144P, Millipore], rabbit anti-α2δ-1 [RRID: AB_258885 , C5105, Sigma-Aldrich]), rabbit anti-α2δ-1 [RRID: AB_2039785 , ACC-015, Alomone Labs], and rabbit anti-GFAP [RRID: AB_10013382 , Z033429-2, Dako] were diluted in 5% NGS or 5% BSA containing PBST.

Techniques: Expressing, Staining, Labeling

Subretinal hUTC transplantation preserves OPL synapses in RCS rats. Representative images of the OPL with the PR ribbon synapses labeled for (A) Bassoon (green) and mGluR6 (red) and (B) Bassoon (green) and α2δ-1 (red) from LE (control), RCS + BSS, and RCS + hUTC P21 and P60 retinas on P95. Quantification of the number of synapses in the OPL revealed that hUTC transplantation protected (C) ribbon synapses. D, Particularly, α2δ-1-containing synapses were specifically preserved following hUTC treatment. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.

Journal: The Journal of Neuroscience

Article Title: Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity

doi: 10.1523/JNEUROSCI.1532-17.2018

Figure Lengend Snippet: Subretinal hUTC transplantation preserves OPL synapses in RCS rats. Representative images of the OPL with the PR ribbon synapses labeled for (A) Bassoon (green) and mGluR6 (red) and (B) Bassoon (green) and α2δ-1 (red) from LE (control), RCS + BSS, and RCS + hUTC P21 and P60 retinas on P95. Quantification of the number of synapses in the OPL revealed that hUTC transplantation protected (C) ribbon synapses. D, Particularly, α2δ-1-containing synapses were specifically preserved following hUTC treatment. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.

Article Snippet: Primary antibodies (mouse anti-Bassoon 1:500 [RRID: AB_10618753 , ADI-VAM-PS003-F, Enzo], rabbit anti-mGluR6 1:150 [RRID:not applicable, RA13105, Neuromics], guinea pig anti-VGlut1 1:750 [AB5905, Millipore], rabbit anti-PSD95 1:500 [RRID: AB_87705 , 51-6900, Invitrogen], mouse anti-Gephyrin 1:250 [RRID: AB_1279448 , 147-021, Synaptic Systems], goat anti-TSP1 1:200 [RRID: AB_2201958 , AF3074, R&D Systems], goat-anti-TSP2 1:200 [RRID: AB_2202068 , AF1635, R&D Systems], mouse anti-glutamine synthetase 1:1000 [RRID: AB_397879 , 610517, BD Biosciences], rabbit anti-SOX9 1:4000 [RRID: AB_2239761 , AB5535, Millipore], goat anti-ChAT[RRID: AB_11214092 , AB144P, Millipore], rabbit anti-α2δ-1 [RRID: AB_258885 , C5105, Sigma-Aldrich]), rabbit anti-α2δ-1 [RRID: AB_2039785 , ACC-015, Alomone Labs], and rabbit anti-GFAP [RRID: AB_10013382 , Z033429-2, Dako] were diluted in 5% NGS or 5% BSA containing PBST.

Techniques: Transplantation Assay, Labeling

Subretinal hUTC transplantation preserves α2δ-1-containing synapses in the IPL of RCS rats. A, Representative images of the IPL labeled for VGluT1 (green) and PSD95 (red) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. B, Quantification of excitatory synapses in the IPL revealed that synapse numbers did not differ between RCS+BSS and RCS+hUTC P21 and P60. C, Representative images of the IPL labeled for Bassoon (green) and α2δ-1 (red) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. D, The α2δ-1-containing synapses were specifically preserved with hUTC transplantation. E, Representative images of the IPL stained for Bassoon (green) and Gephyrin (blue) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. F, hUTC transplantation did not preserve inhibitory synapses. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. *p < 0.05.

Journal: The Journal of Neuroscience

Article Title: Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity

doi: 10.1523/JNEUROSCI.1532-17.2018

Figure Lengend Snippet: Subretinal hUTC transplantation preserves α2δ-1-containing synapses in the IPL of RCS rats. A, Representative images of the IPL labeled for VGluT1 (green) and PSD95 (red) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. B, Quantification of excitatory synapses in the IPL revealed that synapse numbers did not differ between RCS+BSS and RCS+hUTC P21 and P60. C, Representative images of the IPL labeled for Bassoon (green) and α2δ-1 (red) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. D, The α2δ-1-containing synapses were specifically preserved with hUTC transplantation. E, Representative images of the IPL stained for Bassoon (green) and Gephyrin (blue) from LE (control), RCS+BSS, and RCS+hUTC P21 and P60 retinas on P95. F, hUTC transplantation did not preserve inhibitory synapses. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. *p < 0.05.

Article Snippet: Primary antibodies (mouse anti-Bassoon 1:500 [RRID: AB_10618753 , ADI-VAM-PS003-F, Enzo], rabbit anti-mGluR6 1:150 [RRID:not applicable, RA13105, Neuromics], guinea pig anti-VGlut1 1:750 [AB5905, Millipore], rabbit anti-PSD95 1:500 [RRID: AB_87705 , 51-6900, Invitrogen], mouse anti-Gephyrin 1:250 [RRID: AB_1279448 , 147-021, Synaptic Systems], goat anti-TSP1 1:200 [RRID: AB_2201958 , AF3074, R&D Systems], goat-anti-TSP2 1:200 [RRID: AB_2202068 , AF1635, R&D Systems], mouse anti-glutamine synthetase 1:1000 [RRID: AB_397879 , 610517, BD Biosciences], rabbit anti-SOX9 1:4000 [RRID: AB_2239761 , AB5535, Millipore], goat anti-ChAT[RRID: AB_11214092 , AB144P, Millipore], rabbit anti-α2δ-1 [RRID: AB_258885 , C5105, Sigma-Aldrich]), rabbit anti-α2δ-1 [RRID: AB_2039785 , ACC-015, Alomone Labs], and rabbit anti-GFAP [RRID: AB_10013382 , Z033429-2, Dako] were diluted in 5% NGS or 5% BSA containing PBST.

Techniques: Transplantation Assay, Labeling, Staining

KO of MERTK in MG results in impaired synapse development in vivo. A, Representative images of the OPL synapses labeled by Bassoon (green) and mGluR6 (red) from Control (CTR, right eyeballs) and MERTK KO (KO, left eyeballs) at P21 and P45. B, Quantification of synapses in the OPL revealed that synapse development is impaired in MG-specific MERTK-KO at both P21 and P45. C, IPL excitatory synapses are visualized by VGluT1 (green) and PSD95 (red). D, Quantification of synapse number demonstrates that early synaptic development at P21 is significantly affected by MERTK KO but not at P45. Representative images of the (E) OPL and (G) IPL synapses labeled for Bassoon (green) and α2δ-1 (red). Quantification of the number of α2δ-1-containing synapses in both (F) OPL and (H) IPL revealed that KO of MERTK in MG induces severely reduced number of α2δ-1-containing synapses at P21 and P45. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. *p < 0.05.

Journal: The Journal of Neuroscience

Article Title: Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity

doi: 10.1523/JNEUROSCI.1532-17.2018

Figure Lengend Snippet: KO of MERTK in MG results in impaired synapse development in vivo. A, Representative images of the OPL synapses labeled by Bassoon (green) and mGluR6 (red) from Control (CTR, right eyeballs) and MERTK KO (KO, left eyeballs) at P21 and P45. B, Quantification of synapses in the OPL revealed that synapse development is impaired in MG-specific MERTK-KO at both P21 and P45. C, IPL excitatory synapses are visualized by VGluT1 (green) and PSD95 (red). D, Quantification of synapse number demonstrates that early synaptic development at P21 is significantly affected by MERTK KO but not at P45. Representative images of the (E) OPL and (G) IPL synapses labeled for Bassoon (green) and α2δ-1 (red). Quantification of the number of α2δ-1-containing synapses in both (F) OPL and (H) IPL revealed that KO of MERTK in MG induces severely reduced number of α2δ-1-containing synapses at P21 and P45. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. *p < 0.05.

Article Snippet: Primary antibodies (mouse anti-Bassoon 1:500 [RRID: AB_10618753 , ADI-VAM-PS003-F, Enzo], rabbit anti-mGluR6 1:150 [RRID:not applicable, RA13105, Neuromics], guinea pig anti-VGlut1 1:750 [AB5905, Millipore], rabbit anti-PSD95 1:500 [RRID: AB_87705 , 51-6900, Invitrogen], mouse anti-Gephyrin 1:250 [RRID: AB_1279448 , 147-021, Synaptic Systems], goat anti-TSP1 1:200 [RRID: AB_2201958 , AF3074, R&D Systems], goat-anti-TSP2 1:200 [RRID: AB_2202068 , AF1635, R&D Systems], mouse anti-glutamine synthetase 1:1000 [RRID: AB_397879 , 610517, BD Biosciences], rabbit anti-SOX9 1:4000 [RRID: AB_2239761 , AB5535, Millipore], goat anti-ChAT[RRID: AB_11214092 , AB144P, Millipore], rabbit anti-α2δ-1 [RRID: AB_258885 , C5105, Sigma-Aldrich]), rabbit anti-α2δ-1 [RRID: AB_2039785 , ACC-015, Alomone Labs], and rabbit anti-GFAP [RRID: AB_10013382 , Z033429-2, Dako] were diluted in 5% NGS or 5% BSA containing PBST.

Techniques: In Vivo, Labeling

NHE1 colocalisation with CaV1.2 ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.

Journal: Scientific Reports

Article Title: The Na +/ H + -Exchanger NHE1 Regulates Extra- and Intracellular pH and Nimodipine-sensitive [Ca 2+ ] i in the Suprachiasmatic Nucleus

doi: 10.1038/s41598-019-42872-w

Figure Lengend Snippet: NHE1 colocalisation with CaV1.2 ( A ) and NCX1 ( B ), as well as colocalisation of NHE1/CaV1.2 with SERT ( C ), NPY ( D ), and Bassoon ( E ). ( A , B ) High magnification images showing high levels of NHE1 colocalisation with CaV1.2 ( A ), but not NCX1 ( B ). ( C – E ) High magnification images showing moderate to high levels of NHE1/CaV1.2 colocalisation with SERT ( C ) and Bassoon ( E ), but not NPY ( D ). Scale bar: 10 µm.

Article Snippet: For immunofluorescence staining, sections (20 µm) were washed for 20–30 min in PBS and then incubated overnight at 4 °C in PBS containing 2% serum, 0.3% Triton X-100, and primary antibodies against NHE1 (rabbit anti-NHE1; 1:100; ab67314, RRID:AB_1141782; Abcam, Cambridge, MA, USA), neurophysin II (NP2) (goat anti-NP2; 1:500; sc-27093, RRID:AB_2061964; Santa Cruz, CA, USA), vasoactive intestinal peptide (VIP) (guinea pig anti-VIP; 1:500; T-5030, RRID:AB_518690; Peninsula Laboratories, San Carlos, CA, USA), gastrin-releasing peptide (GRP) (goat anti-GRP; 1:100; sc-7788, RRID:AB_2232721; Santa Cruz, CA, USA), vesicular glutamate transporter type 2 (vGluT2) (guinea pig anti-vGluT2; 1:300; AB2251, RRID:AB_1587626; Millipore, Temecula, CA, USA), serotonin transporter (SERT) (mouse anti-SERT; 1:200; MAB1564, RRID:AB_94220; Millipore, Temecula, CA, USA), neuropeptide Y (NPY) (goat anti-NPY; 1:300; NBP1-46535, RRID:AB_10009813; Novus, Littleton, CO, USA), CaV1.2 (guinea pig anti-CaV1.2; 1:100; AGP-001; RRID:AB_11219156; Alomone Labs, Jerusalem, Israel), NCX1 (mouse anti-NCX1, against epitope between amino acid 371 and 525 on intracellular side of plasma membrane; 1:100; AB2869, RRID:AB_2191134; Abcam, MA, USA), and Bassoon (mouse anti-Bassoon; 1:200; ADI-VAM-PS003-D, RRID:AB_2038857; Enzo Life Sciences, Farmingdale, NY, USA).

Techniques: